Title : Downregulation of CACNA2D1 protein is the key event involved in reducing hypertension-induced myocardial injury in exercised mice
Abstract:
Hypertensive?HTN? is one of the risk factors for cardiovascular disease, and exercise has a protective effect on the heart. However, the mechanism by which exercise affects HTN-induced myocardial damage is unclear. In this study, the exercise response model of HTN myocardial injury in mice was analyzed by multi-omics method to determine the possible influencing factors. The results showed that the concentrations of serum Ca2+ and brain natriuretic peptide in HTN group were significantly higher than those in control group, HTN+MICT group and HTN+HIIT group. Myocardial tissue injury and fibrosis increased in HTN group, but exercise training reduced pathological changes, and HTN+HIIT group improved more significantly. Therefore, we speculate that exercise may play a regulatory role in hypertension by altering the expression of certain genes and proteins in the body. The results of omics study showed that there were significant differences in the expression of CACNA2D1 among different treatment groups. HIIT down-regulates CACNA2D1 through exercise, reduces Ca2+ concentration, and dilates vascular smooth muscle. In order to further elucidate the potential molecular mechanism of HIIT's effect on hypertension-induced myocardial damage through downregulation of CACNA2D1 gene, we performed western blot detection on eNOS-NO-cGMP-PKG signaling pathway, and the results showed that mice after HIIT, The expression levels of eNOS and PKG protein were significantly higher than those in HTN group and MICT group, indicating that HIIT can improve the myocardial damage induced by hypertension by down-regulating CACNA2D1 gene. Therefore, our study shows that in exercise therapy, HIIT can significantly treat HTN mice by downregulating CACNA2D1, reducing Ca2+ concentration, and dilating vascular smooth muscle, thereby improving myocardial damage caused by hypertension in mice.
Audience Take Away:
1 Through this study, we can have a new understanding of myocardial damage caused by hypertension, because we provide a new target and idea.
2 This research that other faculty could use to expand their research or teaching, because this study provides new insights through omics testing.
3 It improve the accuracy of a design, can provide new information to assist in a design problem.
