HYBRID EVENT: You can participate in person at Paris, France or Virtually from your home or work.

2nd Edition of International Heart Congress

June 20-22,2024 | Hybrid Event

June 20 -22, 2024 | Paris, France
Heart Congress 2023

How to evaluate rodents’ heart by ultrasonography?

Ana Faustino, Speaker at Heart Congress
CITAB, UEvora, Portugal
Title : How to evaluate rodents’ heart by ultrasonography?


Heart morphology and function may be non-invasively evaluated by ultrasonography. Rodent models have been used to study several diseases. This study aimed to describe the technique for the evaluation of rats’ heart by ultrasonography. The animals should be anesthetized by an intraperitoneal injection of ketamine (37,5 mg/kg) and xylazine (5 mg/kg) and the left region of the thoracic wall should be shaved using a machine clipper. The animals should be placed in left lateral recumbency and ultrasound transmission gel applied in the left thoracic wall. We have used the Logiq P6 apparatus and a high frequency linear probe (10-12 MHz). Parasternal long-axis (LAX), parasternal short-axis (PAS), 4-chamber view and suprasternal views are obtained, using Bmode, M-mode, Power Doppler, and Pulsed Doppler. The images can be recorded in the ultrasound apparatus, and then exported and analysed using Horos, or a similar software. In the LAX images may evaluated the following parameters: diastolic interventricular septal thickness (IVS d), diastolic left ventricular lumen (LV d), diastolic left ventricular posterior wall thickness (LVPW d), systolic interventricular septal thickness (IVS s), left ventricular lumen in systole (LV s), left ventricular posterior wall thickness in systole (LVPW s) and aorta artery diameter (Ao d). Heart rate (HR) and pulmonary artery dimeter (Pa d) can be evaluated in LAX images. In the 4-chamber images can be evaluated the following parameters: left atrial area (LA), right atrial area (AD), tricuspid annulus plane systolic excursion (TAPSE), and E/A wave ratio. Additionally, left ventricular ejection time (LVET) can be evaluated in the suprasternal view.


Ana Faustino is Professor at Department of Zootechnics of University of Évora and Researcher at CITAB/UTAD. She holds a Master in Veterinary Medicine and a European PhD in Veterinary Sciences. Animal models of cancer, tumoral angiogenesis and imaging are her main areas of interest. She has collaborating in several Financed Research projects. The results of her works were published in more than 250 publications in several formats. She received several prizes of scientific merit, and highlights and press honors. She has experience in supervising graduate and post-graduate students. She participated in several courses, workshops, international and national meetings. She is editorial member of several scientific journals and reviewer of several manuscripts. She is Guest Editor of two special issues in Veterinary Animals and in Life.